Composite

Part:BBa_K4719013:Design

Designed by: Auguste Stankeviciute   Group: iGEM23_Vilnius-Lithuania   (2023-09-03)


AGM1-NAG5-UAP1 operon for incorporation of N-acetylglucosamine into bacterial cellulose


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1913
    Illegal EcoRI site found at 3794
    Illegal EcoRI site found at 3956
    Illegal SpeI site found at 37
    Illegal SpeI site found at 2700
    Illegal SpeI site found at 3732
    Illegal SpeI site found at 4620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1913
    Illegal EcoRI site found at 3794
    Illegal EcoRI site found at 3956
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal SpeI site found at 2700
    Illegal SpeI site found at 3732
    Illegal SpeI site found at 4620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1913
    Illegal EcoRI site found at 3794
    Illegal EcoRI site found at 3956
    Illegal BglII site found at 1484
    Illegal BglII site found at 1583
    Illegal BamHI site found at 3204
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1913
    Illegal EcoRI site found at 3794
    Illegal EcoRI site found at 3956
    Illegal SpeI site found at 37
    Illegal SpeI site found at 2700
    Illegal SpeI site found at 3732
    Illegal SpeI site found at 4620
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1913
    Illegal EcoRI site found at 3794
    Illegal EcoRI site found at 3956
    Illegal SpeI site found at 37
    Illegal SpeI site found at 2700
    Illegal SpeI site found at 3732
    Illegal SpeI site found at 4620
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

.


Source

synthetic

References